IDENTIFICATION OF MILK SPECIES BY PROTEIN PROFILE AFTER SDS- PAGE

Human milk as will as non-traditional of protein milk from
species mares', ass's, cow, buffalo, goat, sheep and camel were
investigated by SDS-PAGE polyacrylamide gel electrophoresis. The
results of human milk proteins showed that whey fraction contains,
lactoferrin, secretory IgA (SIgA), serum albumin and α-lactalbumin
which represents 22.8, 10.99, 9.08 and 17.02 respectively. The
investigation will be concern with differentiation of milk species by
protein profile. The majority of proteins in human, mares' and ass's
milk proteins are found in the whey fraction and represented by five
proteins; α-lactalbumin, lactoferrin, Secretory IgA (SIgA), Serum
albumin and Lysozyme. While the major whey proteins in the other
species (cow, buffalo, goat and sheep) represented by four proteins;
lactoferrin, Secretory IgA (SIgA), Serum albumin and β-lactoglobulin.
The major similarity between species were identified between mares'
and ass's as well as human milk proteins especially in the appearance
of lysozyme and α-lactalbumin in high concentration. These
observations encourage the possibility of using these milks as
alternative of human milk