MOLECULAR GENETICS CHARACTERIZATION OF SOME GAMMA RAYS PEANUT MUTANTS

Document Type : Original Article

Abstract

Local peanut cultivars; Giza 4 and Giza 5 were exposed to
different doses (50, 100, 150, 200, 250 and 300 Gy) of Co60 gamma
rays source. All irradiated materials were cultivated to give the M1 and
M2 generations. Then, high yielding mutants were selected from the
M generation and cultivated separately to give the M2 3 generation.
Some yielding component traits were measured for the three
generations (M , M and M ) and M families. The M1 2 3 3 3 selected
mutants (highest yielding mutants and lowest yielding mutants) were
artificially infected by Aspergillus flavus L spores (Aflatoxins - B1
and B2 groups - producer strain). Aflatoxin concentrations were
estimated and the analysis showed that the aflatoxin concentrations in
seeds of the M3 selected mutants from Giza 4 cultivar were higher
than those in seeds of the M3 mutants selected from Giza 5 cultivar.
However, the concentration of aflatoxin was increased when the yield
(weight of seeds per plant trait) increased. RAPD-PCR analysis using
primers A19, A20, B01, B08, C13 and C18 were successfully
differentiated these mutants from their controls and showed high
polymorphism among the M families.

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