GENETIC IDENTIFICATION OF SOME EGYPTIAN VICIA FABA L. CULTIVARS AND THEIR RELATION TO OROBANCHE CRENATA L. TOLERANCE

Ten Egyptian faba bean cultivars were assessed for Orobanche
tolerance in a field trial during the winter season 2002-2003. Two
traits related to Orobanche infection were estimated i.e., number of
spikes/plant and weight of Orobanche stems/plant. Statistical analysis
revealed differences among the studied cultivars in relation to
Orobanche tolerance/susceptibility. The results confirmed that Giza
843 and Misr1 were the most tolerant cultivars, while Giza 643,
Giza716 and Giza717 were the most susceptible ones. SDS-PAGE
and RAPD analysis were used to study the genetic relationships
among the ten faba bean cultivars and to detect some molecular
genetic markers for Orobanche tolerance /susceptibility. Both SDS-
PAGE and RAPD analysis revealed distinctive bands that successfully
determined tolerant from susceptible bean cultivars. Data analyses for
both SDS-PAGE and RAPD-PCR were used to construct a
dendrogram showing the genetic relationships among the faba bean
cultivars and fitted well with their pedigrees.