BIOCHEMICAL STUDIES ON ANTIBIOTIC RESISTANT AND VIRULENCE GENES OF PSEUDOMONAS AERUGINOSA, ISOLATED FROM MASTITIC COWS' MILK AND ITS ALTERATION ON BLOOD BIOCHEMICAL PARAMETERS

The emerged antibiotic resistant bacteria issue is considered as an
important problem that threats human's life including; health and
agriculture affairs. Pseudomonas aeruginosa is one of the bacteria that
affecting milk production and responsible for mastitis in cattle. Out of 50
mastitic milk samples, only 6 samples were found to be positive for
Pseudomonas aeroginousa with an incidence of 12%. The PCR detection
of Pseudiomonas aeroginosa antibiotic resistance gene blaVIM and the
virulence gene toxA, indicated that only 4 isolates showed to be positive
for blaVIM gene. While, all the 6 positive milk Pseudomonas
aeroginousa isolates showed to be positive for toxA gene. The antibiotic
sensitivity results showed that the isolates were resistant to Tetracycline,
Piperacillin and Oxacillin and Piperacillin. However, these isolates were
sensitive to Amikacin, Ceftazidime Cefoperazone and Impenem. Also, the
infected animal serum biochemical parameters when compared to the
healthy uninfected animals (control group), showed an elevation in the
values of total protein, globulin, triglyceride, alanine aminotransferase,
aspartate aminotransferase, uric acid and creatinine. While it showed
decline in the values of albumin, glucose, cholesterol, low density
lipoprotein, and high density lipoprotein.