STUDIES ON PROPAGATION OF ACIDANTHERA MURIELAE PLANTS BY USING TISSUE CULTURE TECHNIQUE

Document Type : Original Article

Abstract

This study was intended to find out a well-defined commercial
production protocol for in vitro propagation of Acidanthera murielae. In
this respect, buds from Acidanthera corms were effectively surface
sterilized using sodium hypochlorite solution at 2% for 20 min. MS
medium supplemented with 2 mg/l IBA (Indole Butyric Acid) was the
best medium for shoot length and number of leaves promotion during the
establishment stage. For further multiplication, MS medium
supplemented with 1 mg/l BA and 2 mg/l Kin formed the highest number
of shoots. For in vitro rooting, MS medium at half strength supplemented
with 2.0 mg/l IBA was suitable for root formation. The plantlets were
transferred to a mixture of peatmoss:sand at a ratio of 1:1 with great
success for ex vitro acclimatization inside the greenhouse conditions.

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